中华航空航天医学杂志

CHINESE JOURNAL OF AEROSPACE MEDICINE

2003年3月第14卷第1期 March 2003,vol.14,No.1

非均匀恒磁场对急性低压缺氧小鼠学习记忆障碍的预防作用

沙建慧 郭宝军 沈楠 黄晶波 孙新奇 朱芙蓉

（132013  吉林市，第四军医大学吉林军医学院）

    【摘要】 目的  探讨非均匀恒磁场N极、S极对急性低压缺氧引起的学习记忆障碍的预防作用。 方法  动物分为对照组、缺氧组、磁场N极+缺氧组和磁场S极+缺氧组。用避暗实验法和自主活动法，分别观察了非均匀恒磁场N极、S极对急性低压缺氧引起的学习记忆障碍的影响。用电镜观察急性低压缺氧及非均匀恒磁场N极、S极作用后，脑皮质细胞形态学改变。结果  ①避暗实验表明：磁场N极+缺氧组、磁场S极+缺氧组与缺氧组相比，记忆成绩M值显著减少(P<0.01或P<0.05)，潜伏期显著延长(P<0.01或P<0.05)。磁场N极+缺氧组与磁场S极+缺氧组相比，前者潜伏期较长(P<0.05)。②缺氧组与其它3组相比，自主活动明显减少(P<0.01)。③非均匀恒磁场可缓解缺氧导致神经元的核变形以及核膜结构不光滑等现象。 结论  非均匀恒磁场对缺氧引起的障碍有预防性作用，可改善由缺氧造成的记忆功能损害，并减轻脑神经元的病理变化，N极作用更为明显。

    【关键词】 低氧； 学习； 记忆； 电磁场； 显微镜检查，电子

    【中图分类号】 R852.11 R454.1

Preventive effect of uneven constant magnetic field on learning and memory impairment induced by acute hypobaric hypoxia in mice  SHA Jian-hui*, GUO Bao-jun, SHEN Nan, et al. *Department of Physiology, Jilin City Military Medical College, Fourth Military Medical University, Jilin 132013, China

【Abstract】 Objective  To investigate preventive effect of uneven constant magnetic field on learning and memory impairment induced by hypobaric hypoxia in mice. Method  Seventy two mice were divided into control group, hypoxia group(8000 m,30 min), magnetic field pole N(15 days)+ hypoxia group and magnetic field pole S(15 days)+ hypoxia group. The stepthrough test and independent activity were used to observe effect of uneven constant magnetic field pole N and S on learning and memory impairment induced by hypobaric hypoxia . The histomorphological changes of brain cortex were observed by electron microscope Results  ① The results of stepthrough test indicated that the mistakes of magnetic field pole N+ hypoxia group and magnetic field pole S+ hypoxia group were less than that of hypoxia group (P<0.01,P<0.05) and the escape latency was longer than that of hypoxia group (P<0.01,P<0.05). Compared with the magnetic field pole S+ hypoxia group, the escape latency of the magnetic field pole N + hypoxia group was longer (P<0.05). ② The independent activity of hypoxia group rats was significantly less than that of other 3 groups (P<0.01).③ Uneven constant magnetic field could alleviate the deformation of neuronal nucleus and change of nuclear membrane structure induced by hypoxia. Conclusions  Staying in constant magnetic field especially pole N possesses preventive effect against impairment of brain induced by acute hypoxia.

【Key words】 Anoxia; Learning; Memory; Electromagnetic fields; Microscopy, electron